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a21209 goat anti dll4 r d systems  (R&D Systems)


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    Structured Review

    R&D Systems a21209 goat anti dll4 r d systems
    A21209 Goat Anti Dll4 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+dll4/Mouse+DLL4+Antibody/10__1016_slash_j__isci__2026__115205-188-47-50
    Average 95 stars, based on 114 article reviews
    a21209 goat anti dll4 r d systems - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Steppogenin suppresses tumor growth and sprouting angiogenesis through inhibition of HIF-1α in tumors and DLL4 activity in the endothelium.
    Article Snippet: Immunohistochemical analyses and hypoxic region determination For immunohistochemical analysis, harvested tumor or tissue samples were fixed in 4% paraformaldehyde and embedded in tissue S. Cha et al. Phytomedicine 108 (2023) 154513 freezing medium (Leica, Wetzlar, Germany). .. The frozen blocks were cut into 10-μm sample sections, which were blocked with 3% BSA in PBST (0.3% Triton X-100 in PBS) and incubated overnight at 4 ◦C with the appropriate primary antibody, which included the following: anti-CD34 (Boster Bio, CA, USA), anti-DLL4 (R&D systems, MN, USA), antipimonidazole (Hypoxyprobe-1, HPI, MA, USA), anti-CD3 (Abcam), anti-CD4 (Invitrogen), and anti-CD8 (Invitrogen). ..

    Article Title: Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells
    Article Snippet: .. The tissue sections were blocked in phosphate-buffered saline containing 0.2% Tween-20 and 5% goat or donkey serum (Jackson ImmunoResearch, West Grove, PA, MD, USA) for 1 h and then incubated overnight with the following primary antibodies: anti-Dll4 (goat; AF1389; R&D Systems, Minneapolis, USA), anti-CD31 (hamster; 2H8; Millipore, Billerica, USA), anti-perilipin (guinea pig; 20R-PP004; Fitzgerald Industries International, Acton, USA), anti-NCAM1 (goat; AF2408; R&D Systems, Minneapolis, USA), anti-Connexin43 (rabbit; 71-0700; Invitrogen, Waltham, USA), anti-FOXP3 (rabbit; ab215206; Abcam, Cambridge, UK), anti-CD3 (rat; MAB4841; R&D Systems, Minneapolis, USA), anti-CD206 (rat; MAB25351; R&D Systems, Minneapolis, USA), and anti-F4/80 (rabbit; SP115; Invitrogen, Waltham, USA). .. After several washes, the sections were incubated for an additional 2 h with the following secondary antibodies: Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-goat IgG, Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-hamster IgG, Alexa Fluor 647-conjugated anti-guinea pig IgG, Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-rabbit IgG, or Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-rat IgG (all secondary antibodies were obtained from Jackson ImmunoResearch, West Grove, USA).

    Immunodetection:

    Article Title: Role of endothelial PDGFB in arterio-venous malformations pathogenesis
    Article Snippet: .. For immunodetection: anti-NG2 (#AB5320, 1:200, millipore), Isolectin B4 (IB4, #121412, 10 μg/ml, Life Technologies), anti-GOLPH4 (#ab28049; 1:200, Abcam), anti-ERG (#92513; 1:200, Abcam), anti-KLF4 (#AF3158, 1:200, R&D systems), anti-SOX17 (#AF1924, 1:200, R&D systems), anti-VE-cadherin (#555289, 1:400, BD), anti-Jag1 (#AF599, 1:200, R&D systems), anti-Endomucin (#sc-65495, 1:200, Santa Cruz), anti-Dll4 (#AF1389, 1:200, R&D systems), anti-phospho-SMAD3 (#ab52903, 1:100, Abcam), anti-phospho-SMAD1/5 (#13820S, 1:100, Cell Signalling), anti-ID1 (#AF4377, 1:100, R&D systems), anti-Endoglin (#AF1320, 1:100, R&D systems), anti-GM130 (#610823; 1:600 BD Bioscience), anti-BMP6 (#ab15640, 1:100, Abcam), anti-TGF-β1 (#MAB7666, 1:100, R&D systems), anti-αSMA (#ab184675,1:600, Abcam), anti-ICAM2 (#553326, 1:200, BD), anti-Collagen IV (#ab6586, 1:200, Abcam) and anti-P21 from CNIO-Centro Nacional de Investigaciones Oncológicas). .. For WB: anti-PPDGFB (#ab23914, 1:1,000, Abcam), anti-SMAD1 (#6944S, 1:1,000, Cell Signalling), anti-SMAD2/3 (#8685S; 1:1000; Cell Signalling), anti-phospho-SMAD1/5/8 (#13820S, 1:1000, Cell Signalling), anti-phospho-SMAD3 (#ab52903, 1:1000, Abcam), anti-TGF-β1 (#sc52893, 1:500, Santa cruz) and anti-VE-cadherin (#sc9989, 1:200, Santa cruz).

    Article Title: Role of endothelial PDGFB in arterio-venous malformations pathogenesis.
    Article Snippet: .. For immunodetection: anti-NG2 (#AB5320, 1:200, millipore), Isolectin B4 (IB4, #121412, 10 μg/ml, Life Technologies), anti-GOLPH4 (#ab28049; 1:200, Abcam), anti-ERG (#92513; 1:200, Abcam), anti-KLF4 (#AF3158, 1:200, R&D systems), anti-SOX17 (#AF1924, 1:200, R&D systems), anti-VE-cadherin (#555289, 1:400, BD), anti-Jag1 (#AF599, 1:200, R&D systems), anti-Endomucin (#sc65495, 1:200, Santa Cruz), anti-Dll4 (#AF1389, 1:200, R&D systems), anti-phospho-SMAD3 (#ab52903, 1:100, Abcam), anti-phospho-SMAD1/5 (#13820S, 1:100, Cell 1 3 Signalling), anti-ID1 (#AF4377, 1:100, R&D systems), antiEndoglin (#AF1320, 1:100, R&D systems), anti-GM130 (#610823; 1:600 BD Bioscience), anti-BMP6 (#ab15640, 1:100, Abcam), anti-TGF-β1 (#MAB7666, 1:100, R&D systems), anti-αSMA (#ab184675,1:600, Abcam), anti-ICAM2 (#553326, 1:200, BD), anti-Collagen IV (#ab6586, 1:200, Abcam) and anti-P21 from CNIO-Centro Nacional de Investigaciones Oncológicas). .. For WB: anti-PPDGFB (#ab23914, 1:1,000, Abcam), anti-SMAD1 (#6944S, 1:1,000, Cell Signalling), antiSMAD2/3 (#8685S; 1:1000; Cell Signalling), anti-phospho-SMAD1/5/8 (#13820S, 1:1000, Cell Signalling), anti-phospho-SMAD3 (#ab52903, 1:1000, Abcam), antiTGF-β1 (#sc52893, 1:500, Santa cruz) and anti-VE-cadherin (#sc9989, 1:200, Santa cruz).

    Labeling:

    Article Title: Sex differences and risk factors for bleeding in Alagille syndrome
    Article Snippet: Retinas were flat‐mounted in Vectashield (cat. no. H‐1000, Vector Laboratories). .. Primary antibodies used: rat anti‐mouse CD31 (RRID: AB_394816, cat. #553370, BD Biosciences, 1:100), rabbit anti‐ERG (RRID: AB_2630401, cat. #ab92513, Abcam, 1:200), goat anti‐DLL4 (RRID: AB_354770, cat. #AF1389, RnD systems, dilution 1:2,000), goat anti‐CD13 (RRID: AB_2227288, cat. #AF2335, RnD Systems, 1:200), goat anti‐Jagged1 (RRID: AB_260348, cat. #J4127, Sigma‐Aldrich, 1:500), mouse anti‐human α‐smooth muscle actin (Cy3 conjugated, RRID: AB_476856, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, RRID: AB_476977, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti‐Myosin heavy chain 11 (RRID: AB_2147146, cat. #ab53219, Abcam, 1:200), goat anti‐Collagen I (RRID: AB_2753206, cat. #1310‐01, Southern Biotech, 1:200), mouse anti‐vimentin (RRID: AB_628437, cat. #sc6360, Santa Cruz, 1:200), rabbit anti‐Transgelin (RRID: AB_443021, cat. #ab14106, Abcam, 1:200), rabbit anti‐HISTONE H3 (phospho S10; RRID: AB_304763, cat. #ab5176, Abcam, 1:500), mouse anti‐GFAP (Cy3 conjugated, RRID: AB_11213580, cat. # MAB3402C3, Millipore, 1:500), rabbit anti‐Cleaved CASPASE3 (RRID: AB_2341188, cat. #9661 (D175), Cell Signaling, 1:700), rabbit anti‐COLLAGEN type IV (RRID: AB_2276457, cat. # AB756P, Millipore, 1:500), mouse anti‐Neurofilament (RRID: AB_528399, cat. #RT97, DSHB, 1:200) nuclei were labeled with DAPI (cat. #D9542, Sigma Aldrich, 1:1,000). .. Images were captured using LSM 510 META, LSM 880, LSM980 (Carl Zeiss AG) microscopes and processed in Image J (NIH), and/or Adobe image suite software (Adobe Inc).

    Saline:

    Article Title: Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells
    Article Snippet: .. The tissue sections were blocked in phosphate-buffered saline containing 0.2% Tween-20 and 5% goat or donkey serum (Jackson ImmunoResearch, West Grove, PA, MD, USA) for 1 h and then incubated overnight with the following primary antibodies: anti-Dll4 (goat; AF1389; R&D Systems, Minneapolis, USA), anti-CD31 (hamster; 2H8; Millipore, Billerica, USA), anti-perilipin (guinea pig; 20R-PP004; Fitzgerald Industries International, Acton, USA), anti-NCAM1 (goat; AF2408; R&D Systems, Minneapolis, USA), anti-Connexin43 (rabbit; 71-0700; Invitrogen, Waltham, USA), anti-FOXP3 (rabbit; ab215206; Abcam, Cambridge, UK), anti-CD3 (rat; MAB4841; R&D Systems, Minneapolis, USA), anti-CD206 (rat; MAB25351; R&D Systems, Minneapolis, USA), and anti-F4/80 (rabbit; SP115; Invitrogen, Waltham, USA). .. After several washes, the sections were incubated for an additional 2 h with the following secondary antibodies: Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-goat IgG, Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-hamster IgG, Alexa Fluor 647-conjugated anti-guinea pig IgG, Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-rabbit IgG, or Alexa Fluor 488- or Alexa Fluor 594-conjugated anti-rat IgG (all secondary antibodies were obtained from Jackson ImmunoResearch, West Grove, USA).

    other:

    Article Title: Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection.
    Article Snippet: Antib-tubulin (SangonBiotech, 1:1000; D410006) and anti-GAPDH (Zen-bio, 1:1000; 380626) antibodies were used for normalization.

    Immunostaining:

    Article Title: Eph-ephrin signaling couples endothelial cell sorting and arterial specification
    Article Snippet: Antibodies used Reported in Supplementary Table 2, 4, 5 Validation Anti-Dach1 (Proteintech 10914-1-AP) antibody has been validated by Proteintech by demonstrating immunostaining on HEK-293 cells. .. Anti-Dll4 (R&D Systems AF1389) antibody has been validated by R&D Systems by immunostaining on bEnd.3 mouse cells and by immunoblotting on bEnd.3 cell lysates. .. Anti-EphB4 (R&D Systems AF446) antibody has been validated by R&D Systems by immunostaining of fixed sections of mouse embryo.

    Western Blot:

    Article Title: Eph-ephrin signaling couples endothelial cell sorting and arterial specification
    Article Snippet: Antibodies used Reported in Supplementary Table 2, 4, 5 Validation Anti-Dach1 (Proteintech 10914-1-AP) antibody has been validated by Proteintech by demonstrating immunostaining on HEK-293 cells. .. Anti-Dll4 (R&D Systems AF1389) antibody has been validated by R&D Systems by immunostaining on bEnd.3 mouse cells and by immunoblotting on bEnd.3 cell lysates. .. Anti-EphB4 (R&D Systems AF446) antibody has been validated by R&D Systems by immunostaining of fixed sections of mouse embryo.



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